Construct: shRNA TRCN0000140632

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015172.2-8164s1c1
DNA Barcode:
GCCAGCCTTTCAGAGGATTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRRC2C (23215)
Gene Description:
proline rich coiled-coil 2C
Transcript:
RefSeq NM_015172.2 (NON-CURRENT)
Match location:
Position 8164 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000140632)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGCCTTTCAGAGGATTAA
Hairpin Sequence:
5'-CCGG-GCCAGCCTTTCAGAGGATTAA-CTCGAG-TTAATCCTCTGAAAGGCTGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGCCTTTCAGAGGATTAACTCGAGTTAATCCTCTGAAAGGCTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGCCTTTCAGAGGATTAACTCGAGTTAATCCTCTGAAAGGCTGGC-3'

Other clones with same target sequence:

  1. TRCN0000343834