Construct: shRNA TRCN0000140667

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024707.2-656s1c1
DNA Barcode:
CTTGAGGCTAAGGCACTGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GEMIN7 (79760)
Gene Description:
gem nuclear organelle associated protein 7
Transcript:
RefSeq NM_024707.2 (NON-CURRENT)
Match location:
Position 656 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000140667)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTGAGGCTAAGGCACTGTAT
Hairpin Sequence:
5'-CCGG-CTTGAGGCTAAGGCACTGTAT-CTCGAG-ATACAGTGCCTTAGCCTCAAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTGAGGCTAAGGCACTGTATCTCGAGATACAGTGCCTTAGCCTCAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTGAGGCTAAGGCACTGTATCTCGAGATACAGTGCCTTAGCCTCAAG-3'

Other clones with same target sequence:

(none)