Construct: shRNA TRCN0000140964

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017748.3-1556s1c1
DNA Barcode:
CCTGCTTCATTGAGTCCTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CWC25 (54883)
Gene Description:
CWC25 spliceosome associated protein homolog
Transcript:
RefSeq NM_017748.3 (NON-CURRENT)
Match location:
Position 1556 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000140964)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGCTTCATTGAGTCCTGAA
Hairpin Sequence:
5'-CCGG-CCTGCTTCATTGAGTCCTGAA-CTCGAG-TTCAGGACTCAATGAAGCAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGCTTCATTGAGTCCTGAACTCGAGTTCAGGACTCAATGAAGCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGCTTCATTGAGTCCTGAACTCGAGTTCAGGACTCAATGAAGCAGG-3'

Other clones with same target sequence:

(none)