Construct: shRNA TRCN0000140997

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_139170.2-1294s1c1
DNA Barcode:
CAAGAAGACAACCAGGGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C16orf71 (146562)
Gene Description:
chromosome 16 open reading frame 71
Transcript:
RefSeq NM_139170.2 (NON-CURRENT)
Match location:
Position 1294 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000140997)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGAAGACAACCAGGGAAAT
Hairpin Sequence:
5'-CCGG-CAAGAAGACAACCAGGGAAAT-CTCGAG-ATTTCCCTGGTTGTCTTCTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGAAGACAACCAGGGAAATCTCGAGATTTCCCTGGTTGTCTTCTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGAAGACAACCAGGGAAATCTCGAGATTTCCCTGGTTGTCTTCTTG-3'

Other clones with same target sequence:

(none)