Construct: shRNA TRCN0000141062

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001001704.1-1846s1c1
DNA Barcode:
CCCACAAGAGAAAGCAGGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FLJ44796 (401209)
Gene Description:
uncharacterized FLJ44796
Transcript:
RefSeq NM_001001704.1 (NON-CURRENT)
Match location:
Position 1846 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000141062)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCACAAGAGAAAGCAGGAAA
Hairpin Sequence:
5'-CCGG-CCCACAAGAGAAAGCAGGAAA-CTCGAG-TTTCCTGCTTTCTCTTGTGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCACAAGAGAAAGCAGGAAACTCGAGTTTCCTGCTTTCTCTTGTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCACAAGAGAAAGCAGGAAACTCGAGTTTCCTGCTTTCTCTTGTGGG-3'

Other clones with same target sequence:

(none)