Construct: shRNA TRCN0000141115

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014887.1-1482s1c1
DNA Barcode:
CCATCAAGATGGGTACAGGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
N4BP2L2 (10443)
Gene Description:
NEDD4 binding protein 2 like 2
Transcript:
RefSeq NM_014887.1 (NON-CURRENT)
Match location:
Position 1482 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000141115)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATCAAGATGGGTACAGGTA
Hairpin Sequence:
5'-CCGG-CCATCAAGATGGGTACAGGTA-CTCGAG-TACCTGTACCCATCTTGATGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATCAAGATGGGTACAGGTACTCGAGTACCTGTACCCATCTTGATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATCAAGATGGGTACAGGTACTCGAGTACCTGTACCCATCTTGATGG-3'

Other clones with same target sequence:

(none)