Construct: shRNA TRCN0000141119

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024491.1-465s1c1
DNA Barcode:
CCAATCAAGAACAACGAGCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CEP70 (80321)
Gene Description:
centrosomal protein 70
Transcript:
RefSeq NM_024491.1 (NON-CURRENT)
Match location:
Position 465 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000141119)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAATCAAGAACAACGAGCTA
Hairpin Sequence:
5'-CCGG-CCAATCAAGAACAACGAGCTA-CTCGAG-TAGCTCGTTGTTCTTGATTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAATCAAGAACAACGAGCTACTCGAGTAGCTCGTTGTTCTTGATTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAATCAAGAACAACGAGCTACTCGAGTAGCTCGTTGTTCTTGATTGG-3'

Other clones with same target sequence:

(none)