Construct: shRNA TRCN0000141139

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015937.2-1911s1c1
DNA Barcode:
CACGAGCCAAATGTGGCATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIGT (51604)
Gene Description:
phosphatidylinositol glycan anchor biosynthesis class T
Transcript:
RefSeq NM_015937.2 (NON-CURRENT)
Match location:
Position 1911 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000141139)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACGAGCCAAATGTGGCATTT
Hairpin Sequence:
5'-CCGG-CACGAGCCAAATGTGGCATTT-CTCGAG-AAATGCCACATTTGGCTCGTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACGAGCCAAATGTGGCATTTCTCGAGAAATGCCACATTTGGCTCGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACGAGCCAAATGTGGCATTTCTCGAGAAATGCCACATTTGGCTCGTG-3'

Other clones with same target sequence:

(none)