Construct: shRNA TRCN0000141260

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138414.1-1004s1c1
DNA Barcode:
CCTCAATGTGGCTCAGAGATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SGF29 (112869)
Gene Description:
SAGA complex associated factor 29
Transcript:
RefSeq NM_138414.1 (NON-CURRENT)
Match location:
Position 1004 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000141260)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCAATGTGGCTCAGAGATA
Hairpin Sequence:
5'-CCGG-CCTCAATGTGGCTCAGAGATA-CTCGAG-TATCTCTGAGCCACATTGAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCAATGTGGCTCAGAGATACTCGAGTATCTCTGAGCCACATTGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCAATGTGGCTCAGAGATACTCGAGTATCTCTGAGCCACATTGAGG-3'

Other clones with same target sequence:

(none)