Construct: shRNA TRCN0000141356

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_053064.2-273s1c1
DNA Barcode:
CCGTTTAGGGAGAAGAAGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GNG2 (54331)
Gene Description:
G protein subunit gamma 2
Transcript:
RefSeq NM_053064.2 (NON-CURRENT)
Match location:
Position 273 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000141356)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTTTAGGGAGAAGAAGTTT
Hairpin Sequence:
5'-CCGG-CCGTTTAGGGAGAAGAAGTTT-CTCGAG-AAACTTCTTCTCCCTAAACGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTTTAGGGAGAAGAAGTTTCTCGAGAAACTTCTTCTCCCTAAACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTTTAGGGAGAAGAAGTTTCTCGAGAAACTTCTTCTCCCTAAACGG-3'

Other clones with same target sequence:

(none)