Construct: shRNA TRCN0000141411

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031294.2-1604s1c1
DNA Barcode:
CCTGAAGATTGACAATCGAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DRC3 (83450)
Gene Description:
dynein regulatory complex subunit 3
Transcript:
RefSeq NM_031294.2 (NON-CURRENT)
Match location:
Position 1604 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000141411)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAAGATTGACAATCGAGA
Hairpin Sequence:
5'-CCGG-CCTGAAGATTGACAATCGAGA-CTCGAG-TCTCGATTGTCAATCTTCAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAAGATTGACAATCGAGACTCGAGTCTCGATTGTCAATCTTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAAGATTGACAATCGAGACTCGAGTCTCGATTGTCAATCTTCAGG-3'

Other clones with same target sequence:

(none)