Construct: shRNA TRCN0000141492

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025250.2-3979s1c1
DNA Barcode:
CCGTGTTCCTAACTACTCCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TTYH3 (80727)
Gene Description:
tweety family member 3
Transcript:
RefSeq NM_025250.2 (NON-CURRENT)
Match location:
Position 3979 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000141492)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTGTTCCTAACTACTCCAT
Hairpin Sequence:
5'-CCGG-CCGTGTTCCTAACTACTCCAT-CTCGAG-ATGGAGTAGTTAGGAACACGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTGTTCCTAACTACTCCATCTCGAGATGGAGTAGTTAGGAACACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTGTTCCTAACTACTCCATCTCGAGATGGAGTAGTTAGGAACACGG-3'

Other clones with same target sequence:

(none)