Construct: shRNA TRCN0000141498

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178126.2-309s1c1
DNA Barcode:
CCTGACATCTCTTCGTCTTGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RETREG3 (162427)
Gene Description:
reticulophagy regulator family member 3
Transcript:
RefSeq NM_178126.2 (NON-CURRENT)
Match location:
Position 309 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000141498)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGACATCTCTTCGTCTTGT
Hairpin Sequence:
5'-CCGG-CCTGACATCTCTTCGTCTTGT-CTCGAG-ACAAGACGAAGAGATGTCAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGACATCTCTTCGTCTTGTCTCGAGACAAGACGAAGAGATGTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGACATCTCTTCGTCTTGTCTCGAGACAAGACGAAGAGATGTCAGG-3'

Other clones with same target sequence:

  1. TRCN0000280781