Construct: shRNA TRCN0000141604

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152765.2-560s1c1
DNA Barcode:
CCAGACATCTCAAGAAGATGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
VXN (254778)
Gene Description:
vexin
Transcript:
RefSeq NM_152765.2 (NON-CURRENT)
Match location:
Position 560 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000141604)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGACATCTCAAGAAGATGA
Hairpin Sequence:
5'-CCGG-CCAGACATCTCAAGAAGATGA-CTCGAG-TCATCTTCTTGAGATGTCTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGACATCTCAAGAAGATGACTCGAGTCATCTTCTTGAGATGTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGACATCTCAAGAAGATGACTCGAGTCATCTTCTTGAGATGTCTGG-3'

Other clones with same target sequence:

(none)