Construct: shRNA TRCN0000141843

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012288.2-2624s1c1
DNA Barcode:
GCAGTAGCTTACGCCTGTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRAM2 (9697)
Gene Description:
translocation associated membrane protein 2
Transcript:
RefSeq NM_012288.2 (NON-CURRENT)
Match location:
Position 2624 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000141843)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTAGCTTACGCCTGTTAT
Hairpin Sequence:
5'-CCGG-GCAGTAGCTTACGCCTGTTAT-CTCGAG-ATAACAGGCGTAAGCTACTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTAGCTTACGCCTGTTATCTCGAGATAACAGGCGTAAGCTACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTAGCTTACGCCTGTTATCTCGAGATAACAGGCGTAAGCTACTGC-3'

Other clones with same target sequence:

(none)