Construct: shRNA TRCN0000141913

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001256.2-2953s1c1
DNA Barcode:
CCAGAGTTTCTTGGTTGCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDC27 (996)
Gene Description:
cell division cycle 27
Transcript:
RefSeq NM_001256.2 (NON-CURRENT)
Match location:
Position 2953 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000141913)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGAGTTTCTTGGTTGCTTT
Hairpin Sequence:
5'-CCGG-CCAGAGTTTCTTGGTTGCTTT-CTCGAG-AAAGCAACCAAGAAACTCTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGAGTTTCTTGGTTGCTTTCTCGAGAAAGCAACCAAGAAACTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGAGTTTCTTGGTTGCTTTCTCGAGAAAGCAACCAAGAAACTCTGG-3'

Other clones with same target sequence:

  1. TRCN0000275426