Construct: shRNA TRCN0000141978

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003760.2-5356s1c1
DNA Barcode:
GACTGCAAATGCCAGTGTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EIF4G3 (8672)
Gene Description:
eukaryotic translation initiation factor 4 gamma 3
Transcript:
RefSeq NM_003760.2 (NON-CURRENT)
Match location:
Position 5356 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000141978)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACTGCAAATGCCAGTGTAAT
Hairpin Sequence:
5'-CCGG-GACTGCAAATGCCAGTGTAAT-CTCGAG-ATTACACTGGCATTTGCAGTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACTGCAAATGCCAGTGTAATCTCGAGATTACACTGGCATTTGCAGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACTGCAAATGCCAGTGTAATCTCGAGATTACACTGGCATTTGCAGTC-3'

Other clones with same target sequence:

(none)