Construct: shRNA TRCN0000142004

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001004054.2-716s1c1
DNA Barcode:
GCGTTAATGTTGCTGGAACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
POTEE (445582)
Gene Description:
POTE ankyrin domain family member E
Transcript:
RefSeq NM_001004054.2 (NON-CURRENT)
Match location:
Position 716 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000142004)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTTAATGTTGCTGGAACAT
Hairpin Sequence:
5'-CCGG-GCGTTAATGTTGCTGGAACAT-CTCGAG-ATGTTCCAGCAACATTAACGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTTAATGTTGCTGGAACATCTCGAGATGTTCCAGCAACATTAACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTTAATGTTGCTGGAACATCTCGAGATGTTCCAGCAACATTAACGC-3'

Other clones with same target sequence:

  1. TRCN0000154676