Construct: shRNA TRCN0000142149
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_152385.1-2075s1c1
- DNA Barcode:
- GCAGTCAACCTAATGGAACAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CLHC1 (130162)
- Gene Description:
- clathrin heavy chain linker domain containing 1
- Transcript:
- RefSeq NM_152385.1 (NON-CURRENT)
- Match location:
- Position 2075 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000142149)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCAGTCAACCTAATGGAACAT
- Hairpin Sequence:
-
5'-CCGG-GCAGTCAACCTAATGGAACAT-CTCGAG-ATGTTCCATTAGGTTGACTGC-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCAGTCAACCTAATGGAACATCTCGAGATGTTCCATTAGGTTGACTGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCAGTCAACCTAATGGAACATCTCGAGATGTTCCATTAGGTTGACTGC-3'
Other clones with same target sequence:
(none)
