Construct: shRNA TRCN0000142199

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032511.1-1870s1c1
DNA Barcode:
GATGGATTCTTGAACCTCCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAXC (84553)
Gene Description:
failed axon connections homolog, metaxin like GST domain containing
Transcript:
RefSeq NM_032511.1 (NON-CURRENT)
Match location:
Position 1870 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000142199)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGGATTCTTGAACCTCCTT
Hairpin Sequence:
5'-CCGG-GATGGATTCTTGAACCTCCTT-CTCGAG-AAGGAGGTTCAAGAATCCATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGGATTCTTGAACCTCCTTCTCGAGAAGGAGGTTCAAGAATCCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGGATTCTTGAACCTCCTTCTCGAGAAGGAGGTTCAAGAATCCATC-3'

Other clones with same target sequence:

(none)