Construct: shRNA TRCN0000142204

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_203299.1-380s1c1
DNA Barcode:
GCCATGTTCTCCTACCAAAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C9orf131 (138724)
Gene Description:
chromosome 9 open reading frame 131
Transcript:
RefSeq NM_203299.1 (NON-CURRENT)
Match location:
Position 380 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000142204)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATGTTCTCCTACCAAAGA
Hairpin Sequence:
5'-CCGG-GCCATGTTCTCCTACCAAAGA-CTCGAG-TCTTTGGTAGGAGAACATGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATGTTCTCCTACCAAAGACTCGAGTCTTTGGTAGGAGAACATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATGTTCTCCTACCAAAGACTCGAGTCTTTGGTAGGAGAACATGGC-3'

Other clones with same target sequence:

(none)