Construct: shRNA TRCN0000142277

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001013739.1-579s1c1
DNA Barcode:
GCCGTCAGATTACTGATACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STAG3L3 (442578)
Gene Description:
stromal antigen 3-like 3 (pseudogene)
Transcript:
RefSeq NM_001013739.1 (NON-CURRENT)
Match location:
Position 579 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000142277)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGTCAGATTACTGATACTT
Hairpin Sequence:
5'-CCGG-GCCGTCAGATTACTGATACTT-CTCGAG-AAGTATCAGTAATCTGACGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGTCAGATTACTGATACTTCTCGAGAAGTATCAGTAATCTGACGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGTCAGATTACTGATACTTCTCGAGAAGTATCAGTAATCTGACGGC-3'

Other clones with same target sequence:

  1. TRCN0000062689
  2. TRCN0000135957