Construct: shRNA TRCN0000142379

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178526.1-702s1c1
DNA Barcode:
GAAGACTCTCTACCATGGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC25A42 (284439)
Gene Description:
solute carrier family 25 member 42
Transcript:
RefSeq NM_178526.1 (NON-CURRENT)
Match location:
Position 702 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000142379)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGACTCTCTACCATGGATT
Hairpin Sequence:
5'-CCGG-GAAGACTCTCTACCATGGATT-CTCGAG-AATCCATGGTAGAGAGTCTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGACTCTCTACCATGGATTCTCGAGAATCCATGGTAGAGAGTCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGACTCTCTACCATGGATTCTCGAGAATCCATGGTAGAGAGTCTTC-3'

Other clones with same target sequence:

(none)