Construct: shRNA TRCN0000142424

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015077.1-1770s1c1
DNA Barcode:
GCAACTTTGTGTTGGTGCTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SARM1 (23098)
Gene Description:
sterile alpha and TIR motif containing 1
Transcript:
RefSeq NM_015077.1 (NON-CURRENT)
Match location:
Position 1770 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000142424)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAACTTTGTGTTGGTGCTAT
Hairpin Sequence:
5'-CCGG-GCAACTTTGTGTTGGTGCTAT-CTCGAG-ATAGCACCAACACAAAGTTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAACTTTGTGTTGGTGCTATCTCGAGATAGCACCAACACAAAGTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAACTTTGTGTTGGTGCTATCTCGAGATAGCACCAACACAAAGTTGC-3'

Other clones with same target sequence:

(none)