Construct: shRNA TRCN0000142571

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021796.1-889s1c1
DNA Barcode:
GATTGGTCTCTTCACACAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PLAC1 (10761)
Gene Description:
placenta enriched 1
Transcript:
RefSeq NM_021796.1 (NON-CURRENT)
Match location:
Position 889 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000142571)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATTGGTCTCTTCACACAGAT
Hairpin Sequence:
5'-CCGG-GATTGGTCTCTTCACACAGAT-CTCGAG-ATCTGTGTGAAGAGACCAATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATTGGTCTCTTCACACAGATCTCGAGATCTGTGTGAAGAGACCAATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATTGGTCTCTTCACACAGATCTCGAGATCTGTGTGAAGAGACCAATC-3'

Other clones with same target sequence:

  1. TRCN0000292335