Construct: shRNA TRCN0000142655

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030814.4-5171s1c1
DNA Barcode:
GCCAGGTTCATCATACCGTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MIR600HG (81571)
Gene Description:
MIR600 host gene
Transcript:
RefSeq NM_030814.4 (NON-CURRENT)
Match location:
Position 5171 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000142655)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGGTTCATCATACCGTTA
Hairpin Sequence:
5'-CCGG-GCCAGGTTCATCATACCGTTA-CTCGAG-TAACGGTATGATGAACCTGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGGTTCATCATACCGTTACTCGAGTAACGGTATGATGAACCTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGGTTCATCATACCGTTACTCGAGTAACGGTATGATGAACCTGGC-3'

Other clones with same target sequence:

(none)