Construct: shRNA TRCN0000142707

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015899.1-1484s1c1
DNA Barcode:
CCCTGAATAACGCATATGGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PLEKHA8P1 (51054)
Gene Description:
pleckstrin homology domain containing A8 pseudogene 1
Transcript:
RefSeq NM_015899.1 (NON-CURRENT)
Match location:
Position 1484 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000142707)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGAATAACGCATATGGTA
Hairpin Sequence:
5'-CCGG-CCCTGAATAACGCATATGGTA-CTCGAG-TACCATATGCGTTATTCAGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGAATAACGCATATGGTACTCGAGTACCATATGCGTTATTCAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGAATAACGCATATGGTACTCGAGTACCATATGCGTTATTCAGGG-3'

Other clones with same target sequence:

(none)