Construct: shRNA TRCN0000142717

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181521.1-667s1c1
DNA Barcode:
CGGCATATGCAGTGAACACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CMTM4 (146223)
Gene Description:
CKLF like MARVEL transmembrane domain containing 4
Transcript:
RefSeq NM_181521.1 (NON-CURRENT)
Match location:
Position 667 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000142717)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCATATGCAGTGAACACAT
Hairpin Sequence:
5'-CCGG-CGGCATATGCAGTGAACACAT-CTCGAG-ATGTGTTCACTGCATATGCCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCATATGCAGTGAACACATCTCGAGATGTGTTCACTGCATATGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCATATGCAGTGAACACATCTCGAGATGTGTTCACTGCATATGCCG-3'

Other clones with same target sequence:

  1. TRCN0000343193