Construct: shRNA TRCN0000142773

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152792.1-963s1c1
DNA Barcode:
CCATATCACCAAGCTAAGGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ASPRV1 (151516)
Gene Description:
aspartic peptidase retroviral like 1
Transcript:
RefSeq NM_152792.1 (NON-CURRENT)
Match location:
Position 963 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000142773)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATATCACCAAGCTAAGGTT
Hairpin Sequence:
5'-CCGG-CCATATCACCAAGCTAAGGTT-CTCGAG-AACCTTAGCTTGGTGATATGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATATCACCAAGCTAAGGTTCTCGAGAACCTTAGCTTGGTGATATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATATCACCAAGCTAAGGTTCTCGAGAACCTTAGCTTGGTGATATGG-3'

Other clones with same target sequence:

(none)