Construct: shRNA TRCN0000142787

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020704.1-3483s1c1
DNA Barcode:
CCCGTTTATTGCTAGCAGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STRIP2 (57464)
Gene Description:
striatin interacting protein 2
Transcript:
RefSeq NM_020704.1 (NON-CURRENT)
Match location:
Position 3483 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000142787)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGTTTATTGCTAGCAGTTT
Hairpin Sequence:
5'-CCGG-CCCGTTTATTGCTAGCAGTTT-CTCGAG-AAACTGCTAGCAATAAACGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGTTTATTGCTAGCAGTTTCTCGAGAAACTGCTAGCAATAAACGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGTTTATTGCTAGCAGTTTCTCGAGAAACTGCTAGCAATAAACGGG-3'

Other clones with same target sequence:

(none)