Construct: shRNA TRCN0000142814

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031459.3-1661s1c1
DNA Barcode:
CCGAAGAATGTACAACCTCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SESN2 (83667)
Gene Description:
sestrin 2
Transcript:
RefSeq NM_031459.3 (NON-CURRENT)
Match location:
Position 1661 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000142814)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGAAGAATGTACAACCTCTT
Hairpin Sequence:
5'-CCGG-CCGAAGAATGTACAACCTCTT-CTCGAG-AAGAGGTTGTACATTCTTCGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGAAGAATGTACAACCTCTTCTCGAGAAGAGGTTGTACATTCTTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGAAGAATGTACAACCTCTTCTCGAGAAGAGGTTGTACATTCTTCGG-3'

Other clones with same target sequence:

(none)