Construct: shRNA TRCN0000142944

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030945.1-276s1c1
DNA Barcode:
CCAGGAAACCATGGAAACAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C1QTNF3 (114899)
Gene Description:
C1q and TNF related 3
Transcript:
RefSeq NM_030945.1 (NON-CURRENT)
Match location:
Position 276 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000142944)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGGAAACCATGGAAACAAT
Hairpin Sequence:
5'-CCGG-CCAGGAAACCATGGAAACAAT-CTCGAG-ATTGTTTCCATGGTTTCCTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGGAAACCATGGAAACAATCTCGAGATTGTTTCCATGGTTTCCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGGAAACCATGGAAACAATCTCGAGATTGTTTCCATGGTTTCCTGG-3'

Other clones with same target sequence:

(none)