Construct: shRNA TRCN0000142954

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001017930.1-2522s1c1
DNA Barcode:
CCAGAGGAGAATTGATGAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DCAF8L1 (139425)
Gene Description:
DDB1 and CUL4 associated factor 8 like 1
Transcript:
RefSeq NM_001017930.1 (NON-CURRENT)
Match location:
Position 2522 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000142954)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGAGGAGAATTGATGAGAA
Hairpin Sequence:
5'-CCGG-CCAGAGGAGAATTGATGAGAA-CTCGAG-TTCTCATCAATTCTCCTCTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGAGGAGAATTGATGAGAACTCGAGTTCTCATCAATTCTCCTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGAGGAGAATTGATGAGAACTCGAGTTCTCATCAATTCTCCTCTGG-3'

Other clones with same target sequence:

(none)