Construct: shRNA TRCN0000142982

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001001790.1-119s1c1
DNA Barcode:
CCATACGGAACTTTCTCATCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TOMM5 (401505)
Gene Description:
translocase of outer mitochondrial membrane 5
Transcript:
RefSeq NM_001001790.1 (NON-CURRENT)
Match location:
Position 119 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000142982)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATACGGAACTTTCTCATCT
Hairpin Sequence:
5'-CCGG-CCATACGGAACTTTCTCATCT-CTCGAG-AGATGAGAAAGTTCCGTATGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATACGGAACTTTCTCATCTCTCGAGAGATGAGAAAGTTCCGTATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATACGGAACTTTCTCATCTCTCGAGAGATGAGAAAGTTCCGTATGG-3'

Other clones with same target sequence:

(none)