Construct: shRNA TRCN0000143120

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001009909.1-169s1c1
DNA Barcode:
CAGGACTATGAAGAGCTAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LUZP2 (338645)
Gene Description:
leucine zipper protein 2
Transcript:
RefSeq NM_001009909.1 (NON-CURRENT)
Match location:
Position 169 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000143120)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGGACTATGAAGAGCTAGAA
Hairpin Sequence:
5'-CCGG-CAGGACTATGAAGAGCTAGAA-CTCGAG-TTCTAGCTCTTCATAGTCCTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGGACTATGAAGAGCTAGAACTCGAGTTCTAGCTCTTCATAGTCCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGGACTATGAAGAGCTAGAACTCGAGTTCTAGCTCTTCATAGTCCTG-3'

Other clones with same target sequence:

(none)