Construct: shRNA TRCN0000143123

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031206.2-1646s1c1
DNA Barcode:
CCATTGGGAAGTCTCCATATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LAS1L (81887)
Gene Description:
LAS1 like ribosome biogenesis factor
Transcript:
RefSeq NM_031206.2 (NON-CURRENT)
Match location:
Position 1646 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000143123)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATTGGGAAGTCTCCATATA
Hairpin Sequence:
5'-CCGG-CCATTGGGAAGTCTCCATATA-CTCGAG-TATATGGAGACTTCCCAATGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATTGGGAAGTCTCCATATACTCGAGTATATGGAGACTTCCCAATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATTGGGAAGTCTCCATATACTCGAGTATATGGAGACTTCCCAATGG-3'

Other clones with same target sequence:

  1. TRCN0000297153