Construct: shRNA TRCN0000143173

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024522.1-1228s1c1
DNA Barcode:
GCCTTGAACACAGTGTACTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NKAIN1 (79570)
Gene Description:
sodium/potassium transporting ATPase interacting 1
Transcript:
RefSeq NM_024522.1 (NON-CURRENT)
Match location:
Position 1228 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000143173)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTGAACACAGTGTACTTT
Hairpin Sequence:
5'-CCGG-GCCTTGAACACAGTGTACTTT-CTCGAG-AAAGTACACTGTGTTCAAGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTGAACACAGTGTACTTTCTCGAGAAAGTACACTGTGTTCAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTGAACACAGTGTACTTTCTCGAGAAAGTACACTGTGTTCAAGGC-3'

Other clones with same target sequence:

(none)