Construct: shRNA TRCN0000143268

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025160.3-992s1c1
DNA Barcode:
GAGGCCATAATGAAGACTTCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WDR26 (80232)
Gene Description:
WD repeat domain 26
Transcript:
RefSeq NM_025160.3 (NON-CURRENT)
Match location:
Position 992 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000143268)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGCCATAATGAAGACTTCA
Hairpin Sequence:
5'-CCGG-GAGGCCATAATGAAGACTTCA-CTCGAG-TGAAGTCTTCATTATGGCCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGCCATAATGAAGACTTCACTCGAGTGAAGTCTTCATTATGGCCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGCCATAATGAAGACTTCACTCGAGTGAAGTCTTCATTATGGCCTC-3'

Other clones with same target sequence:

(none)