Construct: shRNA TRCN0000143281

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015050.1-3181s1c1
DNA Barcode:
GATGCCAAATTGGAGACCATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CMTR1 (23070)
Gene Description:
cap methyltransferase 1
Transcript:
RefSeq NM_015050.1 (NON-CURRENT)
Match location:
Position 3181 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000143281)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGCCAAATTGGAGACCATT
Hairpin Sequence:
5'-CCGG-GATGCCAAATTGGAGACCATT-CTCGAG-AATGGTCTCCAATTTGGCATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGCCAAATTGGAGACCATTCTCGAGAATGGTCTCCAATTTGGCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGCCAAATTGGAGACCATTCTCGAGAATGGTCTCCAATTTGGCATC-3'

Other clones with same target sequence:

  1. TRCN0000297448