Construct: shRNA TRCN0000143306

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001029859.1-108s1c1
DNA Barcode:
GAAGCTCTATACAACCTCACT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KCTD21 (283219)
Gene Description:
potassium channel tetramerization domain containing 21
Transcript:
RefSeq NM_001029859.1 (NON-CURRENT)
Match location:
Position 108 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000143306)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGCTCTATACAACCTCACT
Hairpin Sequence:
5'-CCGG-GAAGCTCTATACAACCTCACT-CTCGAG-AGTGAGGTTGTATAGAGCTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGCTCTATACAACCTCACTCTCGAGAGTGAGGTTGTATAGAGCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGCTCTATACAACCTCACTCTCGAGAGTGAGGTTGTATAGAGCTTC-3'

Other clones with same target sequence:

(none)