Construct: shRNA TRCN0000143366

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014042.1-362s1c1
DNA Barcode:
GAGAAAGACAACAACCTGGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ANAPC15 (25906)
Gene Description:
anaphase promoting complex subunit 15
Transcript:
RefSeq NM_014042.1 (NON-CURRENT)
Match location:
Position 362 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000143366)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGAAAGACAACAACCTGGTT
Hairpin Sequence:
5'-CCGG-GAGAAAGACAACAACCTGGTT-CTCGAG-AACCAGGTTGTTGTCTTTCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGAAAGACAACAACCTGGTTCTCGAGAACCAGGTTGTTGTCTTTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGAAAGACAACAACCTGGTTCTCGAGAACCAGGTTGTTGTCTTTCTC-3'

Other clones with same target sequence:

  1. TRCN0000344428