Construct: shRNA TRCN0000143469

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020751.1-1410s1c1
DNA Barcode:
GCATCTCACGATTCTTCAGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
COG6 (57511)
Gene Description:
component of oligomeric golgi complex 6
Transcript:
RefSeq NM_020751.1 (NON-CURRENT)
Match location:
Position 1410 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000143469)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATCTCACGATTCTTCAGTT
Hairpin Sequence:
5'-CCGG-GCATCTCACGATTCTTCAGTT-CTCGAG-AACTGAAGAATCGTGAGATGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATCTCACGATTCTTCAGTTCTCGAGAACTGAAGAATCGTGAGATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATCTCACGATTCTTCAGTTCTCGAGAACTGAAGAATCGTGAGATGC-3'

Other clones with same target sequence:

  1. TRCN0000319256