Construct: shRNA TRCN0000143485

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178566.2-596s1c1
DNA Barcode:
GAAGGACATATTCCAGGCATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZDHHC21 (340481)
Gene Description:
zinc finger DHHC-type containing 21
Transcript:
RefSeq NM_178566.2 (NON-CURRENT)
Match location:
Position 596 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000143485)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGGACATATTCCAGGCATA
Hairpin Sequence:
5'-CCGG-GAAGGACATATTCCAGGCATA-CTCGAG-TATGCCTGGAATATGTCCTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGGACATATTCCAGGCATACTCGAGTATGCCTGGAATATGTCCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGGACATATTCCAGGCATACTCGAGTATGCCTGGAATATGTCCTTC-3'

Other clones with same target sequence:

  1. TRCN0000278261