Construct: shRNA TRCN0000143492
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_015522.2-169s1c1
- DNA Barcode:
- GATGGAGCTGAAATTGCAGAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DYNC2LI1 (51626)
- Gene Description:
- dynein cytoplasmic 2 light intermediate chain 1
- Transcript:
- RefSeq NM_015522.2 (NON-CURRENT)
- Match location:
- Position 169 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000143492)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GATGGAGCTGAAATTGCAGAA
- Hairpin Sequence:
-
5'-CCGG-GATGGAGCTGAAATTGCAGAA-CTCGAG-TTCTGCAATTTCAGCTCCATC-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGATGGAGCTGAAATTGCAGAACTCGAGTTCTGCAATTTCAGCTCCATCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGATGGAGCTGAAATTGCAGAACTCGAGTTCTGCAATTTCAGCTCCATC-3'
Other clones with same target sequence:
-
TRCN0000353073
