Construct: shRNA TRCN0000143590

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173551.1-848s1c1
DNA Barcode:
GAGCTGGGAATTAAGACAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ANKS6 (203286)
Gene Description:
ankyrin repeat and sterile alpha motif domain containing 6
Transcript:
RefSeq NM_173551.1 (NON-CURRENT)
Match location:
Position 848 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000143590)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCTGGGAATTAAGACAGAT
Hairpin Sequence:
5'-CCGG-GAGCTGGGAATTAAGACAGAT-CTCGAG-ATCTGTCTTAATTCCCAGCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCTGGGAATTAAGACAGATCTCGAGATCTGTCTTAATTCCCAGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCTGGGAATTAAGACAGATCTCGAGATCTGTCTTAATTCCCAGCTC-3'

Other clones with same target sequence:

(none)