Construct: shRNA TRCN0000143692

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015497.2-1719s1c1
DNA Barcode:
GCAGTTAAAGATGGCTACCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TMEM87A (25963)
Gene Description:
transmembrane protein 87A
Transcript:
RefSeq NM_015497.2 (NON-CURRENT)
Match location:
Position 1719 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000143692)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTTAAAGATGGCTACCAT
Hairpin Sequence:
5'-CCGG-GCAGTTAAAGATGGCTACCAT-CTCGAG-ATGGTAGCCATCTTTAACTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTTAAAGATGGCTACCATCTCGAGATGGTAGCCATCTTTAACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTTAAAGATGGCTACCATCTCGAGATGGTAGCCATCTTTAACTGC-3'

Other clones with same target sequence:

(none)