Construct: shRNA TRCN0000143706

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006584.1-1515s1c1
DNA Barcode:
GCAGGAGTTTGGGATAATTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CCT6B (10693)
Gene Description:
chaperonin containing TCP1 subunit 6B
Transcript:
RefSeq NM_006584.1 (NON-CURRENT)
Match location:
Position 1515 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000143706)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGAGTTTGGGATAATTAT
Hairpin Sequence:
5'-CCGG-GCAGGAGTTTGGGATAATTAT-CTCGAG-ATAATTATCCCAAACTCCTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGAGTTTGGGATAATTATCTCGAGATAATTATCCCAAACTCCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGAGTTTGGGATAATTATCTCGAGATAATTATCCCAAACTCCTGC-3'

Other clones with same target sequence:

  1. TRCN0000353642