Construct: shRNA TRCN0000143743

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022831.1-368s1c1
DNA Barcode:
GAATTGCGAAGTGCAGCTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AIDA (64853)
Gene Description:
axin interactor, dorsalization associated
Transcript:
RefSeq NM_022831.1 (NON-CURRENT)
Match location:
Position 368 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000143743)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAATTGCGAAGTGCAGCTTTA
Hairpin Sequence:
5'-CCGG-GAATTGCGAAGTGCAGCTTTA-CTCGAG-TAAAGCTGCACTTCGCAATTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAATTGCGAAGTGCAGCTTTACTCGAGTAAAGCTGCACTTCGCAATTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAATTGCGAAGTGCAGCTTTACTCGAGTAAAGCTGCACTTCGCAATTC-3'

Other clones with same target sequence:

(none)