Construct: shRNA TRCN0000143761

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020817.1-2842s1c1
DNA Barcode:
CTTCGTAGGAAGGTAGTTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CCDC191 (57577)
Gene Description:
coiled-coil domain containing 191
Transcript:
RefSeq NM_020817.1 (NON-CURRENT)
Match location:
Position 2842 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000143761)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCGTAGGAAGGTAGTTGAA
Hairpin Sequence:
5'-CCGG-CTTCGTAGGAAGGTAGTTGAA-CTCGAG-TTCAACTACCTTCCTACGAAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCGTAGGAAGGTAGTTGAACTCGAGTTCAACTACCTTCCTACGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCGTAGGAAGGTAGTTGAACTCGAGTTCAACTACCTTCCTACGAAG-3'

Other clones with same target sequence:

(none)