Construct: shRNA TRCN0000143848

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024941.1-1513s1c1
DNA Barcode:
CCATTGGACTTTGTTCTCCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRAPPC13 (80006)
Gene Description:
trafficking protein particle complex 13
Transcript:
RefSeq NM_024941.1 (NON-CURRENT)
Match location:
Position 1513 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000143848)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATTGGACTTTGTTCTCCAA
Hairpin Sequence:
5'-CCGG-CCATTGGACTTTGTTCTCCAA-CTCGAG-TTGGAGAACAAAGTCCAATGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATTGGACTTTGTTCTCCAACTCGAGTTGGAGAACAAAGTCCAATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATTGGACTTTGTTCTCCAACTCGAGTTGGAGAACAAAGTCCAATGG-3'

Other clones with same target sequence:

(none)